Journal: Brain, behavior, and immunity
Article Title: Microglia-targeted inhibition of miR-17 via mannose-coated lipid nanoparticles improves pathology and behavior in a mouse model of Alzheimer’s disease
doi: 10.1016/j.bbi.2024.05.006
Figure Lengend Snippet: (A) Confocal Microscopy images of microglial ASC in brain sections from cortices of male 5XFAD mice injected with either Scr or Anti-17 MLNPs. Brain sections were stained with microglial marker IBA1 (Green) and ASC (red), and nuclei with DAPI (blue). N = 3 Scr MLNPs and 3 Anti-17 MLNPs injected mice. Unpaired t -test was used for statistical analysis. *P < 0.05 . (B &C) The volume of ASC in the cortex and ASC colocalized with microglia (IBA1) were measured using Imaris software from z-stack images of 5XFAD injected mice brains. N = 3, t -test was used for statistical analysis, *P < 0.05 . (D) ASC Immunoblot of microglia (CD11b+) isolated from male and female 5XFAD injected mice, β-Actin was used as a loading control. N = 3 males and N = 2 females. (E) Relative copy numbers (RCN) of Asc from brains of 5XFAD mice injected with either Scr or Anti-17 MLNPs. N = 3, unpaired t -test was used for statistical analysis, ***P < 0.001 , (F) Immunoblots for ASC from brain homogenates of WT and 5XFAD mice either Anti-17 or Scr MLNPs injected for 4 weeks. M (Male) and F (Female) (G) Densitometry for ASC in F was performed using ImageJ software and normalized to β-Actin. N = 3, One-way ANOVA was used for statistical analysis.
Article Snippet: Membranes were incubated overnight with antibodies against human beta-amyloid (Cell signaling technology, 8243S), mouse ASC (Cell signaling technology, 67824), and GAPDH (Cell Signaling Technology, 2118).
Techniques: Confocal Microscopy, Injection, Staining, Marker, Software, Western Blot, Isolation, Control
